GLS Monotips

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MonoTips based on three different modifications of the monolithic silica are available for specific applications:

MonoTipTM C18		download technical details
MonoTipTM Trypsin		download technical details
MonoTipTM TiO2		download technical details

MonoTips for small scale sample preparation

Sample preparation is a critical procedure in ensuring the successful mass spectrometric analysis of proteins and peptides. Micropipette tips are increasingly being used as a microextraction technique for the purification and enrichment of analytes prior to MALDI-TOF, LC-MS/MS, CE and other techniques. They enable submicrolitre quantities of biological fluids to be handled and the proteins and peptides of interest to be purified and enriched prior to analysis. This includes removing salts and other ion-suppressing interferences from the matrix.

The GL Sciences’ MonoTips are based on monolithic silica with a bimodal structure, consisting of macropores which allow rapid flow of mobile phase and mesopores which form the silica skeleton, creating a large surface area. The unique monolith structure contributes to low pressure-drop and strong analyte-to-surface interactions.

General Features of MonoTips include:
Purification and enrichment of femtomole to micromole levels of peptides and proteins Faster sample preparation High recovery No contamination from monolithic silica Individual tips or 96-well format

MonoTipTM C18
Use for sample concentration, desalting and removal of detergents


Monolithic silica properties and chemical modification
Silica type - High purity sol-gel silica gel Specific surface area - 200m2/g Through pore size - 10~20μm Meso pore size - 20nm Bonded phase - Octadecyl group Carbon load - 12% Tip volume - 200μL Sample capacity - 100μg (Angiotensin II) Application scope (Molecular weight) - ~40,000

MonoTipTMmini C18
Use for sample concentration, desalting and removal of detergents

Monolithic silica properties and chemical modification
Silica type - High purity sol-gel silica gel Specific surface area - 200m2/g Through pore size - 20~320μm Meso pore size - 15nm Bonded phase - Octadecyl group Carbon load - 12% Tip volume - 10μL Sample capacity - 5μg (Angiotensin II) Application scope (Molecular weight) - ~5,000


MonoTip TM Trypsin
Use for digestion of proteins in proteomic analysis (enzyme action effected by aspirating and expelling 10x at room temperature)

Monolithic silica properties and chemical modification
Tip volume 200µl Large sample capacity (100µg protein)

MonoTip TM TiO2
Use for purification and concentration of phosphorylated peptides

Tip volume 200µl Sample capacity 15µg (tyrosine phosphopeptide)

Prices for these MonoTips are available here. For further information on these MonoTips or for details of alternative phases in pipette tip format, please contact Hichrom.

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